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POLAR DISTRIBUTION OF RADIANT INTENSITIES
The representation of a light beam such as an electromagnetic wave or a collection of photons is very useful to understand how light interacts with matter. However, in describing light in the atmosphere, we are primarily interested in the flow of energy.+++ ...
The UVC inactivation mechanism of microorganisms causes cellular damage by inducing changes in the chemical structure of DNA chains. The result is the production of cyclo butane pyrimidine (CPD) dimers causing a distortion of the DNA molecule, which causes dysfunctions in cell replication and leads to cell death.
spectral distribution in the UV range
Ultraviolet (UV) irradiation is an electromagnetic irradiation whose wavelength (100-400 nm) is shorter than that of visible light (400-700 nm), but longer than X-rays < (100 nm). UVC irradiation (200-280 nm).+++ ...
The UVC inactivation mechanism of microorganisms damages genetic material in the nucleus of the cell or nucleic acids in the virus.
The UVC spectrum, especially the range of 250 to 270 nm, is heavily absorbed by the nucleic acids of a microorganism and, therefore, is the deadliest wavelength range for microorganisms. This range, with 262 nm being the maximum germicidal wavelength, is known as the germicidal spectrum. , this often produces a defect that prevents the microorganism from being viable.
Dosage of exposure to ultraviolet rays
The degree of inactivation by ultraviolet rays is directly related to the UV dose applied.
the dose is the product of the UV intensity [I] (expressed in energy per unit area) and the exposure time [T].
Therefore: DOSE = I x T+++ ...
This dose, sometimes called fluence, is commonly measured in milli-joules per square centimeter (mJ/cm2). “J/m2” units are used in most parts of the world.
The reduction of microorganisms is classified using a logarithmic scale. A single logarithmic reduction corresponds to a 90% reduction in organisms. A reduction of two logs corresponds to a 99% reduction in organisms, followed by a reduction of three logs (99.9%), etc.